IFN-γ Negatively Modulates Self-Renewal of Repopulating Human Hemopoietic Stem Cells

IFN- Negatively Modulates Self-Renewal of Repopulating Human Hemopoietic Stem Cells

Self-Renewal of Stem Cells

Asymmetric division is one of the most fundamental characteristics of adult stem cells , which ensures one daughter cell maintains stem cell status and the other daughter cell becomes committed to differentiation. New data emerged recently that allow us to conclude that asymmetric division has another important aspect: it enables self-maintenance of stem cells.

Separate control of the survival, the self-renewal and the differentiation of hemopoietic stem cells.

Hemopoietic stem cells adhere to hemopoietic supportive (MS-5) cells, but not to non-supportive (MS-K) cells. Although a soluble stem cell factor (SCF) was produced by both of these cell lines, little activity was detectable in the supernatant from the cultures of either of these cells, indicating that SCF might be compartmentalized within the extracellular matrix (ECM), and transferred directl...

Physical Separation of Hemopoietic Stem Cells Differing in Their Capacity for Self-renewal

Bone marrow cells in suspension were separated into a number of fractions on the basis of cell size by sedimentation at unit gravity through gradients of fetal calf serum. The colony forming units (CFU) from the various fractions were tested for their self-renewal capacity using a double transplantation technique. The results indicate that the CFU in the fractions containing slowly sedimenting ...

Self-renewal of hemopoietic stem cells during mixed colony formation in vitro.

Replating experiments have shown that the self-renewal of pluripotent hemopoietic stem cells can be studied in vitro by clonal analysis techniques. The number of daughter stem cells detectable in individual primary clones produced in vitro varies markedly from one clone to another. These findings are consistent with a general model of stem cell differentiation in which the choice to self-replic...